MYOD1 inhibits avian adipocyte differentiation via miRNA-206/KLF4 axis

Abstract Background A considerable number of muscle development-related genes were differentially expressed in the early stage avian adipocyte differentiation. However, functions them differentiation remain largely known. In this study, myoblast determination protein 1 ( MYOD1 ) was selected as a representative development. We investigated its expression, function, and regulation Results The expression decreased significantly CRISPR/Cas9-mediated deletion induced differentiation, whereas over-expression inhibited adipogenesis. mRNA-seq data showed that could perturb lipid biosynthetic process during Our results directly up-regulates miR-206 by binding upstream 1200 bp region miR-206. Then, can inhibit Furthermore, affected endogenous target gene Kruppel-like factor 4 KLF4 ), which is an important activator Accordingly, inhibition or counteract inhibitory effect on Conclusions establish inhibits up-regulating to suppress expression. These findings identify novel function suggesting potential role body-fat distribution regulation.